Project VII

Studying vesicular content and trafficking in osteoclasts using advanced imaging and proteomic techniques.

Arjen Gebraad

I am Arjen and I am from Capelle aan den IJssel in the Netherlands. I did my bachelor and master in Biomedical Engineering at the University of Twente, Enschede, the Netherlands. In 2013, I graduated at the Department of Biomaterials Science and Technology at the University of Twente on polymer 3D scaffolds seeded with mesenchymal stem cells for the repair of intervertebral disc lesions.

Since August 2014, I am working as an Early Stage Researcher at the Department of Cell Biology and Anatomy at the University of Turku, Finland.

Tiina Laitala-Leinonen

I am Tiina and I did my PhD-work 1996 on the pH regulation in bone-resorbing osteoclasts, using e.g. antisense molecules to alter resorption activity. Since then, I have worked on short inhibitory and regulatory RNA molecules (siRNAs, miRNAs) in rat, mouse and human hematopoietic and mesenchymal stem cells and primary bone and cartilage cells.

In addition to skeletal tissues, I am also interested in tissue regeneration after sport-induced trauma. We collaborate with scientists, athletes, coaches and clinicians to understand how differentiation of individual cell types could experimentally be regulated.

These experiments are undertaken in order to produce specific cell types for use in e.g. regenerative medicine.


Our Project

In the EUROCLAST project we study the extensive vesicular trafficking in bone-resorbing osteoclasts. We want to understand, how the enzymes and acids used by osteoclasts to resorb bone are produced and transported.

We also study how osteoclasts transcytose bone degradation products from the resorption site to be secreted into the blood stream.

For these purposes, the genetics involved in setting up and maintaining the osteoclast resorption machinery is investigated in detail. In silico -derived data on the proteins involved in vesicular trafficking is verified in vitro by using state-of-the-art microscopy techniques.

Skeletal Biology Consortium (SBC)
Department of Cell Biology and Anatomy
Institute of Biomedicine
University of Turku
Kiinamyllynkatu 10
20520 Turku, Finland
crede.utu.fi

Arjen Gebraad
E: awhgeb[at]utu.fi

Adj. Prof. Tiina Laitala-Leinonen, PhD
E: tilale[at]utu.fi